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magnetic beads  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec magnetic beads
    Magnetic Beads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 98/100, based on 365 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd14+magnetic+beads/CD14+MicroBeads%2C+human+-+lyophilized/pm42497858-391-9-12
    Average 98 stars, based on 365 article reviews
    magnetic beads - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Isolation:

    Article Title: Peripheral nerve injury-induced remodeling of the tumor-associated macrophages promotes immune evasion in breast cancer
    Article Snippet: .. For generation of MDMs, monocytes were isolated from human PBMC using CD14 magnetic beads (Miltenyi, 130-050-201) and resuspended in DMEM containing 20 ng/mL M-CSF (Novoprotein, C417) and 10% FBS. ..

    Article Title: Peripheral nerve injury-induced remodeling of the tumor-associated macrophages promotes immune evasion in breast cancer.
    Article Snippet: .. For generation of MDMs, monocytes were isolated from human PBMC using CD14 magnetic beads (Miltenyi, 130-050-201) and resuspended in DMEM containing 20 ng/mL M-CSF (Novoprotein, C417) and 10% FBS. ..

    Article Title: Recognition of Brucella abortus drives M2 like polarization and impaired antigen presentation in monocyte derived macrophages.
    Article Snippet: Peripheral blood mononuclear cells (PBMCs) were isolated from the buffy coats of healthy anonymous blood donors via density gradient centrifugation using Ficoll‒Hypaque (#171440-03, GE Healthcare, USA). .. Monocytes were isolated from PBMCs via CD14 magnetic beads (#130-097-052, Miltenyi Biotec, USA). .. Only monocytes with a purity of up to 85% were used for the experiments, as determined by flow cytometry with an anti-human CD14-FITC AR TIC LE I PR ES S antibody (#555397, BD Pharmingen, USA) 40.

    Article Title: Recognition of Brucella abortus drives M2 like polarization and impaired antigen presentation in monocyte derived macrophages
    Article Snippet: Peripheral blood mononuclear cells (PBMCs) were isolated from the buffy coats of healthy anonymous blood donors via density gradient centrifugation using Ficoll‒Hypaque (#171440-03, GE Healthcare, USA). .. Monocytes were isolated from PBMCs via CD14 magnetic beads (#130-097-052, Miltenyi Biotec, USA). .. Only monocytes with a purity of up to 85% were used for the experiments, as determined by flow cytometry with an anti-human CD14-FITC antibody (#555397, BD Pharmingen, USA) .

    Article Title: Targeting the Notch1‐YY1‐ICAM1 Signaling Axis Enhances the Efficacy of Immunotherapy in HCC by Activating CD8 + T‐Cell‐Driven Cancer Cell Pyroptosis
    Article Snippet: Peripheral blood mononuclear cells (PBMCs) derived from healthy donors were isolated with Ficoll (Stemcell, Cat. no. 0 7861) by performing density gradient centrifugation at room temperature. .. Monocytes were isolated from PBMCs by using anti‐CD14 magnetic beads according to the manufacturer's protocol (Miltenyi Biotec, Cat. No. 130‐050‐201). .. For the generation of mature dendritic cells (DCs), monocytes were cultured and induced in conditioned medium (RPMI‐1640 medium containing 10% FBS, 1% penicillin/streptomycin, 55 μm β‐mercaptoethanol, 500 U mL −1 IL‐4 (PeproTech, Cat. no. 200‐04‐20), 800 U mL −1 GM‐CSF (PeproTech, Cat. no. 300‐03‐20)) for 5 days, and 20 ng mL −1 TNF‐α (Sino Biological, Cat. no. 10602‐R10N1‐200) was then added to the culture for 2 days to induce DC maturation.

    Magnetic Beads:

    Article Title: Peripheral nerve injury-induced remodeling of the tumor-associated macrophages promotes immune evasion in breast cancer
    Article Snippet: .. For generation of MDMs, monocytes were isolated from human PBMC using CD14 magnetic beads (Miltenyi, 130-050-201) and resuspended in DMEM containing 20 ng/mL M-CSF (Novoprotein, C417) and 10% FBS. ..

    Article Title: Peripheral nerve injury-induced remodeling of the tumor-associated macrophages promotes immune evasion in breast cancer.
    Article Snippet: .. For generation of MDMs, monocytes were isolated from human PBMC using CD14 magnetic beads (Miltenyi, 130-050-201) and resuspended in DMEM containing 20 ng/mL M-CSF (Novoprotein, C417) and 10% FBS. ..

    Article Title: Multivariate Screening and Automated Clustering of Macrophage Immunoreactome to Nanoparticles and Photothermal Therapy
    Article Snippet: PBMCs were isolated by density gradient centrifugation using Lymphoprep (STEMCELL, #0 7851) from peripheral blood leukocyte separation medium. .. Monocytes were then enriched from the PBMC population using CD14+ magnetic beads (Miltenyi Biotec, #130‐050‐201) following the manufacturer's instructions. ..

    Article Title: Recognition of Brucella abortus drives M2 like polarization and impaired antigen presentation in monocyte derived macrophages.
    Article Snippet: Peripheral blood mononuclear cells (PBMCs) were isolated from the buffy coats of healthy anonymous blood donors via density gradient centrifugation using Ficoll‒Hypaque (#171440-03, GE Healthcare, USA). .. Monocytes were isolated from PBMCs via CD14 magnetic beads (#130-097-052, Miltenyi Biotec, USA). .. Only monocytes with a purity of up to 85% were used for the experiments, as determined by flow cytometry with an anti-human CD14-FITC AR TIC LE I PR ES S antibody (#555397, BD Pharmingen, USA) 40.

    Article Title: Recognition of Brucella abortus drives M2 like polarization and impaired antigen presentation in monocyte derived macrophages
    Article Snippet: Peripheral blood mononuclear cells (PBMCs) were isolated from the buffy coats of healthy anonymous blood donors via density gradient centrifugation using Ficoll‒Hypaque (#171440-03, GE Healthcare, USA). .. Monocytes were isolated from PBMCs via CD14 magnetic beads (#130-097-052, Miltenyi Biotec, USA). .. Only monocytes with a purity of up to 85% were used for the experiments, as determined by flow cytometry with an anti-human CD14-FITC antibody (#555397, BD Pharmingen, USA) .

    Article Title: Targeting the Notch1‐YY1‐ICAM1 Signaling Axis Enhances the Efficacy of Immunotherapy in HCC by Activating CD8 + T‐Cell‐Driven Cancer Cell Pyroptosis
    Article Snippet: Peripheral blood mononuclear cells (PBMCs) derived from healthy donors were isolated with Ficoll (Stemcell, Cat. no. 0 7861) by performing density gradient centrifugation at room temperature. .. Monocytes were isolated from PBMCs by using anti‐CD14 magnetic beads according to the manufacturer's protocol (Miltenyi Biotec, Cat. No. 130‐050‐201). .. For the generation of mature dendritic cells (DCs), monocytes were cultured and induced in conditioned medium (RPMI‐1640 medium containing 10% FBS, 1% penicillin/streptomycin, 55 μm β‐mercaptoethanol, 500 U mL −1 IL‐4 (PeproTech, Cat. no. 200‐04‐20), 800 U mL −1 GM‐CSF (PeproTech, Cat. no. 300‐03‐20)) for 5 days, and 20 ng mL −1 TNF‐α (Sino Biological, Cat. no. 10602‐R10N1‐200) was then added to the culture for 2 days to induce DC maturation.

    Article Title: Lipid nanoparticle delivery of circle RNA vaccine induces potent immune responses
    Article Snippet: .. CD14 + magnetic beads (MicroBeads, Miltenyi, 130-050-201) were added at a ratio of 20 μl per 107 PBMCs and incubated at 4 °C in the dark for 15 min. ..

    Article Title: Lipid nanoparticle delivery of circle RNA vaccine induces potent immune responses.
    Article Snippet: .. CD14+ magnetic beads (MicroBeads, Miltenyi, 130-050-201) were added at a ratio of 20 μl per 107 PBMCs and incubated at 4°C in the dark for 15 minutes. ..

    Incubation:

    Article Title: Lipid nanoparticle delivery of circle RNA vaccine induces potent immune responses
    Article Snippet: .. CD14 + magnetic beads (MicroBeads, Miltenyi, 130-050-201) were added at a ratio of 20 μl per 107 PBMCs and incubated at 4 °C in the dark for 15 min. ..

    Article Title: Lipid nanoparticle delivery of circle RNA vaccine induces potent immune responses.
    Article Snippet: .. CD14+ magnetic beads (MicroBeads, Miltenyi, 130-050-201) were added at a ratio of 20 μl per 107 PBMCs and incubated at 4°C in the dark for 15 minutes. ..



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